Clik here to view.
Microscopy
The Compound Microscope Figure 1 There would be little to do in a microbiology laboratory without a microscope, because the objects of our attention (bacteria, fungi, and other single celled creatures)...
View ArticleIntroduction
Course Description for Microbiology Laboratory Manual This course for community college students was designed to be taught as a face to face course that meets twice per week over an 11 week term at...
View ArticleClik here to view.
Laboratory Safety
In this course you will actually get to manipulate living things and see what happens…exciting! Working with living microorganisms also carries with it the risk of being harmed by these organisms. An...
View ArticleLab Safety Assignment
Lab Safety Assignment: Name: ______________ 1. Sketch the lab space and note the locations of the following: fire extinguisher, slide disposal, incubator, fume hoods, biohazard trash, eye wash,...
View ArticleClik here to view.
Aseptic Transfer
Learning Objectives The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply...
View ArticleClik here to view.
Aseptic Transfer Assignment
Data and Observations Name_______________ Organism Medium form Description of growth before mixing Description of growth after mixing Relative amount of growth 0-5 (0=none, 5=very turbid)...
View ArticleClik here to view.
Streaking for Isolation
Learning Objectives.: The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply...
View ArticleGram Stain Assignment
Name: _______________ Data and Observations Organism color Gram Reaction Drawing Morphology and Arrangement Instructor Initials Post Lab Questions Explain the major differences between the Gram...
View ArticleClik here to view.
Gram Stain
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Use the bright field light...
View ArticleClik here to view.
Streaking for Isolation Assignment
Name ______________________ Sample source Area (1, 2 or 3) where isolated colonies are found Number of different looking colonies Is there evidence of contamination? Your T-streak Evaluation isolated...
View ArticleClik here to view.
Colony Morphology
The student will: Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology regarding microbial growth, when making observations. On agar plates, bacteria...
View ArticleClik here to view.
Colony Morphology Assignment
Name:____________________ What microscopic cell shape(s) might produce a convex colony? What microscopic cell shape(s) might produce filamentous colonies?
View ArticleClik here to view.
SIM Medium
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology...
View ArticleSIM Medium Assignment
Name ______________________ Data and Observations Sulfur Reduction Organism (write name out fully) Observations (include color and location) Result +/- Interpretation Motility Organism (may properly...
View ArticleClik here to view.
Antibiotic Susceptibility Testing
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology...
View ArticleClik here to view.
Antibiotic Susceptibility Assignment
Name _____________ Data and Observations S: Susceptible R: Resistent Post Lab Questions What is the difference between a confluent lawn and confluent growth as seen in the first or second area of a...
View ArticleCatalase Test Assignment
Name _______________ Data and Observations Organism Observations Result +/- Interpretation Post Lab Questions What was the positive control? What was the negative control? 2. What two things about the...
View ArticleClik here to view.
Catalase Test
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology...
View ArticleClik here to view.
Oxidase Test
The student will: Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology regarding microbiological techniques, instruments biochemical testing when...
View ArticleOxidase Test Assignment
Name Lab Section Data and Observations Organism Observations Result +/- Interpretation Post Lab Questions Where is the reagent in this test? 2. The fact that the CRA will spontaneously give up its...
View ArticleClik here to view.
MSA, MacConkey, EMB Plates
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology...
View ArticleMSA, MacConkey, EMB Plates Assignment
Name Lab Section Data and Observations Organism Growth on EMB +/- Appearance on EMB Growth on MacC +/- Appearance on MacC Growth on MSA +/- Appearance on MSA Post Lab Questions Based on your...
View ArticleClik here to view.
Blood Agar
The student will: Use aseptic techniques in the safe inoculation of various forms of media. Follow oral and written instructions and manage time in the lab efficiently. Apply correct terminology...
View ArticleBlood Agar Assignment
Name Lab Section Data and Observations Organism Growth on Blood agar +/- Appearance of surface streak on blood agar Appearance of the stab on blood agar Post Lab Questions Is blood agar selective,...
View ArticleClik here to view.
Acid-Fast and Endospore Staining
Objectives Learn about microorganisms that have acid-fast cell walls. Perform the acid-fast staining procedure and view cells under oil immersion. Learn about some of the microorganisms that are...
View ArticleClik here to view.
Acid Fast and Endospore Staining Assignment
Name:____________ Review Questions Distinguish between the Ziehl-Neelson and Kinyoun methods of acid-fast staining. Why is it important to leave the malachite green on the slide for at least 10...
View ArticleClik here to view.
Dichotomous Keys
Objectives Understand the uses of dichotomous keys. Using the test results throughout this course construct a dichotomous key for the identification of a bacterial unknown. Introduction Keys that are...
View ArticleClik here to view.
Triple Sugar Iron Medium
Triple Sugar Iron is a slant medium with two growth environments: aerobic (on the slant) and anaerobic (in the “butt”). The medium contains three sugars in varying concentrations and a pH...
View ArticleClik here to view.
Microscopy Assignment
The human mouth is home to numerous microbes, which persist no matter how many times you brush your teeth and use mouthwash. Since these microbes generally inhabit the surface layers of the oral...
View ArticleClik here to view.
Chemical Control of Microbial Growth
Objectives: In this lab you will: Learn about disinfectants and the various factors that need to be considered when choosing a disinfectant. Set up an experiment to determine the effectiveness of five...
View ArticleClik here to view.
Chemical Control of Microbial Growth Assignment
Name: _______________ Disinfectant experiment: Use the spaces below to sketch the appearance of your own Nutrient Agar plate. Record your observations below. For the second table, do some research...
View ArticleClik here to view.
Growth Patterns in Broth
Growing bacteria in culture requires consideration of their nutritional and physical needs. Food, provided in the media, is broken down by cells and used for energy and building biomass. Unlike...
View ArticleGrowth Patterns in Broth Assignment
Bacteria Growth Pattern Oxygen Needs What other factors, besides growth in media, are important to record about your microbes? Why? 2. You have two tubes of media that have been inoculated for 24...
View ArticleClik here to view.
Triple Sugar Iron Medium Assignment
https://www.austincc.edu/microbugz/triple_sugar_iron_agar.ph Organism Color and Gas Result Symbol Interpretation (include enzyme when known) Control Questions: You return to evaluate your table after...
View Article